To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 1’s plasmid is;

#Question id: 32343

#Unit 2. Cellular Organization

#Question id: 17674

#Unit 8. Inheritance Biology

#Question id: 4103

#Unit 3. Fundamental Processes

In a biochemical experiment, you compare the products from splicing reactions carried out in vitro using three different substrates. In each case the substrate is a construct containing a single intron surrounded by two exons, and in all cases the construct is the same overall size. But in one case, the intron is a group I intron, in another a group II intron, and in the third an intron removed by the spliceosome. Each construct is labelled in a manner that allows it to be detected after gel electrophoresis, and each is tested in two reactions—one, conditions that support self-splicing, and two, in the presence of nuclear extract as well. Note that, for simplification, only the final products of the splicing reaction are seen, but before degradation of the introns.

 Which of the following observation is/are correct?

A. In lane A, the presence of nuclear abstract, a band for the lariat and the spliced product and pre-mRNA.

B. Lane B shows Group I Introns and Lane C is for Group II introns and reaction is nonself-splicing

C. Lane C is Group I intron. Splicing takes place in the absence and presence of nuclear abstract, and the reaction is self-splicing.

#Question id: 11727

#Unit 6. System Physiology – Plant

The stages of gibberellin biosynthesis takes place various organelles and completed in various forms such as;

a) In the endoplasmic  reticulum GA12 is converted to GA53 by hydroxylation at C-13

b) In the cytosol GA12 is converted via 13-hydroxylation pathway into GA9 and further converted into GA4 by 3-β-Hydroxylation

c) In the cytosol GA53 is converted via 13-Hydroxylation pathway into GA20 and further converted into GA1 by 3-β-Hydroxylation

d) Hydroxylation at C-2 then converts GA4 and GA1 to the inactive forms GA34 and GA8, respectively

Which one of the following given combination from the above statements is true?

#Question id: 7756

#Unit 5. Developmental Biology