To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?

#Question id: 4374

#Unit 3. Fundamental Processes

When a repressor protein is __ (bound, unbound) to a promoter region transcription is allowed; when an activator protein is __ (bound, unbound) to a promoter transcription is ,stimulated.

#Question id: 4375

#Unit 3. Fundamental Processes

Protein X is found to bind to a certain geneʹs promoter only when substance A is not present.  The gene can be transcribed in the presence of both X and A. It is not transcribed in the presence of X alone. Protein X is a/an __. Substance A is a/an __.

#Question id: 4376

#Unit 3. Fundamental Processes

Which of the following is the correct order of binding of general transcription factors to initiate transcription at RNA polymerase II promoters?

#Question id: 4377

#Unit 3. Fundamental Processes

What is the function of TFIIH in the transcription initiation complex?

#Question id: 4378

#Unit 3. Fundamental Processes

This serves as the promoter for 70% of eukaryotic genes and typically serves as a control region for genes that are transcribed at relatively low rates.

#Question id: 4379

#Unit 3. Fundamental Processes

Bromodomains are found in chromosome-associated proteins that contribute to transcriptional activation. To facilitate this activation, the bromodomains bind to histones, specifically their lysine residues that have been post-translationally modified by: