To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?

TLS Online TPP Program

#Question id: 24435

#Unit 1. Molecules and their Interaction Relevant to Biology

Which of the following factors dictates to makes ensure the rotation of the c ring is essentially unidirectional.

TLS Online TPP Program

#Question id: 24436

#Unit 1. Molecules and their Interaction Relevant to Biology

As the number of rings increasing in C subunit per NADH yield of ATP

TLS Online TPP Program

#Question id: 24437

#Unit 1. Molecules and their Interaction Relevant to Biology

If bovine mitochondria have 8 c subunits per c ring, what is the predicted ratio of ATP formed per NADH oxidized?

TLS Online TPP Program

#Question id: 24438

#Unit 1. Molecules and their Interaction Relevant to Biology

The molecular structure of the mitochondrial ATP synthase has been determined by

TLS Online TPP Program

#Question id: 27226

#Unit 1. Molecules and their Interaction Relevant to Biology

The Protein Components of the Mitochondrial Electron-Transfer Chain match them correctly with their prosthetic groups 

TLS Online TPP Program

#Question id: 27941

#Unit 1. Molecules and their Interaction Relevant to Biology

Some of the following statement are made regarding the Enzymes of glycolysis along with their Coenzyme (s) or Cofactor (s) and Inhibitor (s)

a. Phosphoglucoisomerase having Mg2+ as Cofactor and can be inhibited by 2-deoxyglucose 6-phosphate

b. Aldolase having Zn2+ as Cofactor, can be inhibited by Chelating agents

c. Enolase having Mg, Mn22+ Cd2 as Cofactor, can be inhibited by Fluoride + phosphate

d. Phosphofructokinase having NAD as Coenzyme can be inhibited by Iodoacetate

Which of the following is correct