To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?

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#Question id: 7487

#General Aptitude

At what time between 4 and 5 are the hands 2 minute spaces apart?

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#Question id: 1815

#Unit 4. Cell Communication and Cell Signaling

Genuine adaptive T- and B-cell responses are seen in:

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#Question id: 8714

#Unit 9. Diversity of Life Forms

The reason that paralogous genes can diverge from each other within the same gene pool, whereas orthologous genes diverge only after gene pools are isolated from each other, is that ________.

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#Question id: 26471

#Unit 3. Fundamental Processes

Intrinsic terminators are responsible for prokaryotic transcription termination, so which of the following is the characteristics of Intrinsic terminators

a. It  include palindromic regions 

b. It do not include palindromic regions 

c. Palindromic regions that form hairpins varying in length from 7 to 20 bp

d. The stem-loop structure includes a G-C–rich region and is followed by a run of 7U residues.

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#Question id: 29568

#Unit 6. System Physiology – Plant

Pathogen class, with their Species, along with Effector name, and Effector function  are given

Match them correctly