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TLS Online TPP Program

#Question id: 13107

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 
You do each PCR reaction on chromosome 15 and load each one into a separate well of an agarose gel, and then run the gel.
 

 
Why is it that some people show two bands?

#Unit 13. Methods in Biology
  1. The more repeats you have, the longer your DNA fragment is, and longer DNA fragments migrate more slowly in gels.
  2. They are homozygotes for the allele that cannot be cut.
  3. They are homozygotes for the allele that can be cut.
  4. They are heterozygotes, and possess one allele that can be cut and one allele that cannot be cut.
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TLS Online TPP Program

#Question id: 2835

#Unit 2. Cellular Organization

An enhancer

a. can be located upstream of a promoter.

b. can be located downstream of a promoter.

c. can be located a variable distance from the promoter.

d. is always located within 1 kb of the promoter.

e. can be cell-type-specific.
TLS Online TPP Program

#Question id: 2836

#Unit 2. Cellular Organization

The fact that a specific protein leaves a “footprint” on a DNA molecule is indicative of
a. a lack of interaction between the specific protein and DNA.
b. protection from DNAse by the specific protein.
c. binding of the specific protein to all types of DNA.
d. binding of the specific protein to a specific sequence of DNA.
TLS Online TPP Program

#Question id: 2837

#Unit 2. Cellular Organization

The C-terminal activation domain of transcriptional activators is capable of
a. binding to DNA.                 
b. stimulating transcription.
c. interaction with other transcriptional machinery.
d. functioning in a fusion with a DNA-binding domain from an unrelated transcriptional activator.
TLS Online TPP Program

#Question id: 2838

#Unit 2. Cellular Organization

You want to study the potential interaction between nucleosome-bound DNA and a specific histone deacetylase. You decide to perform an electrophoretic mobility shift assay (EMSA). You use a 32P end-labelled, linear template DNA that contains two nucleosome positioning sites. You assemble two nucleosomes on the DNA template before incubation with and without the histone deacetylase. For some reactions, you use unmodified nucleosomes. For other reactions, you use nucleosomes that are methylated at lysine 36 of the histone protein H3.

A. The histone deacetylase binds nucleosome bound-DNA in lanes 1, 2, 3, and 4.

B. The histone deacetylase binds nucleosome bound-DNA in lanes 3& 4.

C. The histone deacetylase seems to recognize methylated nucleosomes at lysine 36 of histone H3 in lane 1, 2 & 3 better than unmethylated nucleosomes in lane 4 &5

D. The histone deacetylase seems to recognize methylated nucleosomes at lysine 36 of histone H3 in lane 1 & 2 better than unmethylated nucleosomes in lane 3 &4
TLS Online TPP Program

#Question id: 2839

#Unit 2. Cellular Organization

Consider for correct match

I. All the different members of family of genes and proteins are sufficiently similar in sequence to suggest a common ancestral sequence.

A. Homologous

II. Sequences that presumably diverged as a result of gene duplication

B. Paralogous

III. Sequences that arose because of speciation

C. Orthologous

TLS Online TPP Program

#Question id: 2840

#Unit 2. Cellular Organization

An experimental setup provided the assay for identifying factors that facilitate transcription in the presence of chromatin. A factor called FACT (facilitates chromatin transcription) functions as