TLS Online TPP Program

#Question id: 13162


In the amoeba Dictyostelium discoideum, cAMP acts as an extracellular signalling molecule and binds to and signals via a G protein–coupled receptor; it is not a second messenger. Amoeba cells were transfected with genes encoding two fusion proteins: a Gα fused to cyan fluorescent protein (CFP), and a Gβ fused to yellow fluorescent protein (YFP). CFP normally fluoresces 490-nm light; YFP, 527-nm light. In which conditions fluorescence
energy transfer occur from CFP to YFP?

#Unit 4. Cell Communication and Cell Signaling
  1. If ligand binding occur it leads to association of the Gα and Gβγ subunits, then fluorescence energy transfer can occur.
  2. If ligand binding occur it leads to dissociation of the Gα and Gβγ subunits, then fluorescence energy transfer can occur.
  3. If ligand binding doesn’t occur (In resting state) it leads to dissociation of the Gα and Gβγ subunits, then fluorescence energy transfer can occur.
  4. If ligand binding doesn’t occur (In resting state) it leads to association of the Gα and Gβγ subunits, then fluorescence energy transfer can occur.
     
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TLS Online TPP Program

#Question id: 3579

#Unit 8. Inheritance Biology

Following some statement is correct with monohybrid and Dihybrid cross?

A) Two alleles for gene segregate into gametes in equal proportions.

B) Monohybrid F2 phenotype 1:2:1 ratio conclude, there is no segregation in character

C)  Monohybrid F2 phenotype 3:1 ratio conclude, there is a no blending inheritance

D) Dihybrid F 2 phenotype 3:1 ratio conclude complete linkage

TLS Online TPP Program

#Question id: 3051

#Unit 2. Cellular Organization

You have 10^10 cell/L of bacterial stock solution. To prepare 1 liter of growth medium containing 1000 bacteria, the volume in microlitre of this stock solution that should be added is

TLS Online TPP Program

#Question id: 24548

#Unit 9. Diversity of Life Forms

Many phenotypic traits are genetically variable within species, they can readily evolve by
a. natural selection
b. adaptation
c. genetic drift
d. mutation

TLS Online TPP Program

#Question id: 5295

#Unit 8. Inheritance Biology

A cross is made between an Hfr that is met+ thi+ pur+ and an F-that is met- thi- pur-. Interrupted mating studies show that met+ enters the recipient last, so met+ recombinants are selected on a medium containing supplements that satisfy only the pur and thi requirements.  These recombinants are tested for the presence of the thi+ and pur+ alleles. The following numbers of individuals are found for each genotype:

A) Gene Order

I

Thi Pur  Met

II

Pur  Met  Thi

III

Pur   Thi  Met

B) Distance between Pur and Thi

IV

15.4 map unit

V

1.8 map unit

What is the following correct combination?

TLS Online TPP Program

#Question id: 20290

#Unit 7. System Physiology – Animal

What are the factors that inhibits glucagon secretion?