Which of the following plant materials is commonly used for mechanical isolation of protoplasts?

TLS Online TPP Program

#Question id: 38761

#Plant Biotechnology

Q. You need to culture a very low density of protoplasts, specifically looking for rare mutants or hybrids. According to the data, which technique is most suitable for supporting growth at low densities? 

TLS Online TPP Program

#Question id: 38762

#Plant Biotechnology

Q. Which method allows for the easiest adjustment of osmotic pressure and density reduction during the culture process? 

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#Question id: 38763

#Plant Biotechnology

Q. Based on the chemical properties of PEG, how does it facilitate the link between the membrane and external ions? 

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#Question id: 38764

#Plant Biotechnology

Q. What is the primary cause of membrane "disturbance" during the PEG-induced fusion process? 

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#Question id: 38765

#Plant Biotechnology

Q. Why is the "washing process" considered a critical step for actual fusion to occur?

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#Question id: 38766

#Plant Biotechnology

Q. Statement 1 (Assertion): Protoplasts located in close proximity are more likely to undergo fusion during the washing phase of a PEG treatment. 

Statement 2 (Reason): The removal of PEG molecules disrupts the organized structure of the plasma lemma, creating localized instabilities that facilitate membrane merging

Which of the following is correct?