Sieving effect that contributes to the separation of proteins according to their size, in techniques such as SDS–gel electrophoresis, the concentration of gel used

TLS Online TPP Program

#Question id: 39280

#Plant Biotechnology

Q. Regarding cybridization for male sterile line development:

Statement I: Cybrids allow rapid transfer of mitochondria even across sexually incompatible species.

Statement II: Male sterile lines developed via protoplast fusion can be used for hybrid seed production using CMS restorers.

Statement III: Nuclear genomes are exchanged between donor and recipient protoplasts in cybridization.

Which statements are correct?

TLS Online TPP Program

#Question id: 39281

#Plant Biotechnology

Q. Statement I: Conventional backcrossing is a more time-efficient method for cytoplasm transfer compared to somatic hybridization. 

Statement II: Protoplast fusion can achieve cytoplasm transfer in approximately one year.

TLS Online TPP Program

#Question id: 39282

#Plant Biotechnology

Q. Statement I: In the cybrid approach, iodoacetate is used to inactivate the nuclei of the recipient strain (Parent A). 

Statement II: Irradiation is used to inactivate the nuclei of the donor strain (Parent B) to ensure only its cytoplasm is transferred.

TLS Online TPP Program

#Question id: 39283

#Plant Biotechnology

Q. Statement I: In the fusion product described, the mitochondria are always derived from the donor strain (Parent B).

 Statement II: The chloroplasts in the resulting regenerants must always come from the recipient strain (Parent A)

TLS Online TPP Program

#Question id: 39284

#Plant Biotechnology

Q. Statement I: Fusing tomato protoplasts with irradiated Solanum acaule protoplasts produced plants that were morphologically identical to Solanum acaule. 

Statement II: The resulting tomato regenerants exhibited Cytoplasmic Male Sterility (CMS), which was inherited maternally.

TLS Online TPP Program

#Question id: 39285

#Plant Biotechnology

Q. Statement I: Protoplast fusion can be used to develop male sterile lines even between sexually incompatible species. 

Statement II: CMS lines developed through this method cannot be used with traditional CMS restorers.