Practice Questions

TLS Online TPP Program

#Question id: 38066

Which of the following statements regarding "Direct Somatic Embryogenesis" is INCORRECT?

#Plant Biotechnology

Embryos regenerate directly from the explant tissue without an intermediate callus phase.
It involves "Pre-Embryonic Determined Cells" (PEDCs).
The cells require extensive reprogramming and dedifferentiation to become embryogenic.
Scutellum of cereals is a common example of an explant used for this pathway.

More Questions

TLS Online TPP Program

#Question id: 37926

#Plant Biotechnology

Arrange the following steps involved in enzymatic isolation, culture, and regeneration of plant protoplasts in the correct sequence:

A. Enzymatic digestion of cell wall using cellulase and pectinase

B. Culturing of isolated protoplasts by hanging drop / feeder layer / agar methods

C. Surface sterilization and epidermis removal from leaf explant

D. Density gradient centrifugation to separate viable protoplasts

E. Callus formation followed by regeneration of a complete plant

Choose the correct order:

TLS Online TPP Program

#Question id: 37927

#Plant Biotechnology

Protoplasts require osmotic stabilizers in culture media because they:

TLS Online TPP Program

#Question id: 37928

#Plant Biotechnology

Which of the following is a non-ionic osmotic stabilizer commonly used in protoplast culture?

TLS Online TPP Program

#Question id: 37929

#Plant Biotechnology

Mannitol is preferred for leaf mesophyll protoplasts because it is:

TLS Online TPP Program

#Question id: 37930

#Plant Biotechnology

The commonly used concentration range of sugar solutions for osmotic stabilization of protoplasts is:

TLS Online TPP Program

#Question id: 37931

#Plant Biotechnology

Which of the following solutes is considered a metabolizable osmotic stabilizer?