At a fraternity party a 17-year-old male places a paper bag over his mouth and breathes in and out of the bag. As he continues to breathe into this bag, his rate of breathing continues to increase. Which of the following is responsible for the increased ventilation?

TLS Online TPP Program

#Question id: 38770

#Plant Biotechnology

Q. Statement I: PEG-induced fusion is a consequence of controlled membrane damage and reorganization.

Statement II: Plasma membrane destabilization increases the likelihood of fusion between closely placed protoplasts.

TLS Online TPP Program

#Question id: 38771

#Plant Biotechnology

Q. Statement I: PEG can interact with both negatively and positively charged components of the plasma membrane.

Statement II: Such interactions reduce electrostatic repulsion between protoplasts.

TLS Online TPP Program

#Question id: 38772

#Plant Biotechnology

Q. Statement I: Protoplasts are isolated by enzymatic digestion of the cell wall using a combination of cellulase and pectinase.

Statement II: Cellulose microfibrils and pectic substances are major structural components of plant cell walls.

TLS Online TPP Program

#Question id: 38773

#Plant Biotechnology

Q.Statement I: The pH of the enzyme solution for protoplast isolation is maintained between 4.7 and 8.0.

Statement II: Enzymes involved in cell wall degradation show activity only at neutral pH.

TLS Online TPP Program

#Question id: 38774

#Plant Biotechnology

Q. Statement I: Protoplast isolation is usually carried out at a temperature range of 25–30°C.

Statement II: This temperature range supports optimal enzyme activity and maintains protoplast viability.

TLS Online TPP Program

#Question id: 38775

#Plant Biotechnology

Q. Statement I: High osmotic concentration of the enzyme mixture is maintained using sorbitol or mannitol.

Statement II: Elevated osmoticum prevents protoplast bursting by balancing internal turgor pressure.