TLS Online TPP Program

#Question id: 40212


Read the following statements regarding the steps of a standard PCR cycle and choose the correct option.

Statement I: The denaturation step involves the breaking of phosphodiester bonds to separate the double-stranded DNA into single strands.

Statement II: The annealing temperature is usually set 3 -5 0   C lower than the melting temperature (Tm) of the primers to ensure specific binding.

#Applied Microbiology
  1. Both Statement I and Statement II are correct.
  2. Both Statement I and Statement II are incorrect.
  3. Statement I is correct, but Statement II is incorrect.
  4. Statement I is incorrect, but Statement II is correct.