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TLS Online TPP Program

#Question id: 4726

Which of the following is incorrect regards genomic imprinting?

#Part-A Aptitude & General Biotechnology

  1. Certain mutations showing lethality if the mutant gene is derived from one parent, but that same mutant gene has no deleterious effects if inherited from the other parent

  2. Genes on autosomal chromosomes that are more readily expressed in one sex

  3. Transgenic male passes an imprinted gene to his offspring, they express the gene; but, when his daughter transmits the same gene to her offspring, they don’t express it

  4. Genes whose expression is affected by the sex of the transmitting parent

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TLS Online TPP Program

#Question id: 13079

#Part-A Aptitude & General Biotechnology

Name of that sophisticated equipment which used to the oligonucleotide, that serve as a primer in RAPDs is usually obtained by in vitro DNA synthesis,
TLS Online TPP Program

#Question id: 13080

#Part-A Aptitude & General Biotechnology

In RAPDs amplification will take place only of those regions of the genome that have the 
TLS Online TPP Program

#Question id: 13081

#Part-A Aptitude & General Biotechnology

Correct statements about RAPD’s
A. RAPD polymorphism is detected by using oligonucleotides usually more than 10 bases long of random sequences as primers in a reaction.
B. In a strain which has in genomic DNA sequences complementary to the primer oligonucleotide, PCR products will be detected in the gel,
C. Typical RAPD markers show limited variation between parents, especially in naturally inbreeding species.
D. RAPDs are more sensitive than RFLPs to experimental conditions making them more difficult to be consistent and reproducible.
TLS Online TPP Program

#Question id: 13082

#Part-A Aptitude & General Biotechnology

Statement: AFLP shares some features of both RFLP and RAPD analyses.
Explanations: I. It uses restriction enzyme-digested genomic DNA as template for PCR amplification using primers that contain the restriction enzyme recognition sites plus a number of, usually 2-3, arbitrary nucleotides.
II. AFLPs are faster, less labour intensive and provide more information than RFLPs, and they are highly reproducible, which is a great advantage over RAPDs.
TLS Online TPP Program

#Question id: 13084

#Part-A Aptitude & General Biotechnology

Statement: In a modified version of AFLP, the selection of fragments using biotin-streptavidin binding is avoided.
Explanations: I. The fragments are ligated to the appropriate adapters and used for PCR amplification using two AFLP primers, each primer having a single selection nucleotide; this is called preamplification step.
II. The PCR products from this preamplificated step are diluted and used as template for second PCR amplification.
TLS Online TPP Program

#Question id: 13085

#Part-A Aptitude & General Biotechnology

The most common microsatellite sequence encountered in human genome is the sequence