TLS Online TPP Program

#Question id: 4813


You perform the three different two-factor crosses (Cross 1: XY and xy, Cross 2: YZ and yz, and Cross 3: XZ and xz). Assume all crosses are between diploid flies homozygous for the alleles of these genes. You observe 7% recombinants in the first cross, 20% recombinants in the second cross, and 13% recombinants in the third cross. if cross between XyZ and xYz leads to which of the followining recombinant with double cross over .

#Part-A Aptitude & General Biotechnology
  1. xyZ      

  2. XyZ        

  3. xyz                    

  4. xYZ

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TLS Online TPP Program

#Question id: 14216

#Part-B Specialized Branches in Biotechnology

The viscosity of fluid decreases with increasing stirrer speed, the fluid would be 

TLS Online TPP Program

#Question id: 14217

#Part-B Specialized Branches in Biotechnology

In a microbial system how true yields are related to apparent growth yield? 

TLS Online TPP Program

#Question id: 14218

#Part-B Specialized Branches in Biotechnology

In CSTR system at steady rate  

TLS Online TPP Program

#Question id: 14218

#Part-A Aptitude & General Biotechnology

In CSTR system at steady rate  

TLS Online TPP Program

#Question id: 14221

#Part-B Specialized Branches in Biotechnology

E. coli have a maximum respiration rate, qO2max, of about 240-mg O2/g-dry wt-h. It is desired to achieve a cell mass of 20 g dry wt/l. The kLa is 120 h-1 in a 1000-l reactor (800 l working volume). A gas stream enriched in oxygen is used (i.e., 80% O2) which gives a value of C* = 28 mg/L. If oxygen becomes limiting, growth and respiration slow; for example,

                                     
   where CL is the dissolved oxygen concentration in the fermenter. What is CL when the cell mass is  at 20 g/l?

TLS Online TPP Program

#Question id: 14237

#Part-B Specialized Branches in Biotechnology

A genetically-engineered strain of yeast is cultured in a bioreactor at 30⁰C for production of heterologous protein. The oxygen requirement is 80 mmol 1-1 h-I; the critical oxygen concentration is 0.004 mM. The solubility of oxygen in the fermentation broth is estimated to be 10% lower than in water due to solute effects. What is the minimum mass-transfer coefficient necessary to sustain this culture if the reactor is sparged with air at approximately 1 atm pressure ?