If the DG'° of the reaction A -> B is –40 kJ/mol, under standard conditions the reaction:

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#Question id: 13060

#Part-A Aptitude & General Biotechnology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

In blue white screening insertional activation of Beta-D-galactosidase will give rise to

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#Question id: 14218

#Part-A Aptitude & General Biotechnology

In CSTR system at steady rate  

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#Question id: 14124

#Part-A Aptitude & General Biotechnology

which of the following database is the first established sequence pattern database?

TLS Online TPP Program

#Question id: 13056

#Part-A Aptitude & General Biotechnology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

 Which of the following is true for traditional and real time PCR?

TLS Online TPP Program

#Question id: 14430

#Part-B Specialized Branches in Biotechnology

Which is not correctly match, effect of process variables on mass transfer characteristics?

Process variable                     mass transfer characteristics

P. P/V                              -          oxygen transfer rate

q. Impeller tip speed     -          mixing time

r. Reynolds                     -          number shear rate