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TLS Online TPP Program
#Question id: 13054
#Part-A Aptitude & General Biotechnology
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?
TLS Online TPP Program
#Question id: 5316
#Part-B Specialized Branches in Biotechnology
Why is sequencing of eukaryotic genomes more difficult than sequencing genomes of bacteria or archaea?
TLS Online TPP Program
#Question id: 5394
#Part-A Aptitude & General Biotechnology
The average score of a cricketer for 20 match is 42.5 runs . If the average for the first 13 matches is 55, then find the average for the Last 7 matches
TLS Online TPP Program
#Question id: 15773
#Part-A Aptitude & General Biotechnology
A series of symmetric and asymmetric cell divisions, called as
TLS Online TPP Program
#Question id: 9285
#Part-B Specialized Branches in Biotechnology
Non-O2-evolving (anoxygenic) organisms, contain only a single photosystem such as