If the experimental population of E. coli lacks an F factor or F plasmid, and if bacteriophage are excluded from the bacterial cultures, then which of these is a means by which beneficial mutations might be transmitted horizontally to other E. coli cells?

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#Question id: 13082

#Part-A Aptitude & General Biotechnology

Statement: AFLP shares some features of both RFLP and RAPD analyses.

Explanations: I. It uses restriction enzyme-digested genomic DNA as template for PCR amplification using primers that contain the restriction enzyme recognition sites plus a number of, usually 2-3, arbitrary nucleotides.

II. AFLPs are faster, less labour intensive and provide more information than RFLPs, and they are highly reproducible, which is a great advantage over RAPDs.

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#Question id: 13084

#Part-A Aptitude & General Biotechnology

Statement: In a modified version of AFLP, the selection of fragments using biotin-streptavidin binding is avoided.

Explanations: I. The fragments are ligated to the appropriate adapters and used for PCR amplification using two AFLP primers, each primer having a single selection nucleotide; this is called preamplification step.

II. The PCR products from this preamplificated step are diluted and used as template for second PCR amplification.

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#Question id: 13085

#Part-A Aptitude & General Biotechnology

The most common microsatellite sequence encountered in human genome is the sequence 

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#Question id: 13153

#Part-A Aptitude & General Biotechnology

The in-radius of an equilateral triangle is of length 3 cm. Then the length of each of its medians is

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#Question id: 13196

#Part-A Aptitude & General Biotechnology

 In The Mica Experiment Dnase I enzyme cleaves 

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#Question id: 13197

#Part-A Aptitude & General Biotechnology

In helical nature of DNA is its periodicity each base pair is displaced (twisted) from the previous one by