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TLS Online TPP Program
#Question id: 13055
#Part-A Aptitude & General Biotechnology
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following will provide least specific amplification in qPCR?
TLS Online TPP Program
#Question id: 14137
#Part-B Specialized Branches in Biotechnology
Consider the following alignment of two DNA sequences:
AGTAAC
AA_ _AC
Assuming an affine gap scoring scheme of an identity matrix for substitution, a gap initiation penalty of 1 and a gap extension penalty of 0.1, the score of the alignment is _________
TLS Online TPP Program
#Question id: 10337
#Part-B Specialized Branches in Biotechnology
In biological nitrogen fixation, the process of nitrification by organism respective bacteria A--which convert the ammonia to nitrite and B--- further converted into nitrate in the soil by their respective A and B bacteria known as;
TLS Online TPP Program
#Question id: 4150
#Part-A Aptitude & General Biotechnology
Posttranslational glycosylation of proteins is inhibited specifically by:
TLS Online TPP Program
#Question id: 5312
#Part-B Specialized Branches in Biotechnology
Why is it more difficult to identify eukaryotic genes than prokaryotic genes using genomic techniques?