TLS Online TPP Program
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TLS Online TPP Program
#Question id: 13057
#Part-A Aptitude & General Biotechnology
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
How primer concentration leads to decrease in PCR efficiency?
TLS Online TPP Program
#Question id: 3857
#Part-A Aptitude & General Biotechnology
The CAAT box is usually located near the position:
TLS Online TPP Program
#Question id: 18833
#Part-A Aptitude & General Biotechnology
The PCR procedure, where Taq polymerase is added after the rest of the PCR components are heated to the DNA melting temperature, so as to avoid non-specific amplification at lower temperatures is
TLS Online TPP Program
#Question id: 1673
#Part-A Aptitude & General Biotechnology
N-region insertion is associated with the expression of:
TLS Online TPP Program
#Question id: 15002
#Part-A Aptitude & General Biotechnology
If you performed a dihybrid cross and found that your results were significantly different from the 9:3:3:1 ratio, what would you suspect?