A certain bacterial mRNA is known to represent only one gene and to contain about 800 nucleotides.  If you assume that the average amino acid residue contributes 110 to the peptide molecular weight, the largest polypeptide that this mRNA could code for would have a molecular weight of about:

#Question id: 13074

#Part-A Aptitude & General Biotechnology

#Question id: 13075

#Part-A Aptitude & General Biotechnology

#Question id: 13076

#Part-A Aptitude & General Biotechnology

Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.

#Question id: 13077

#Part-A Aptitude & General Biotechnology

#Question id: 13078

#Part-A Aptitude & General Biotechnology

#Question id: 13079

#Part-A Aptitude & General Biotechnology