TLS Online TPP Program

#Question id: 4368


Which statement is false concerning the general transcription factor, TFIID?

#Part-A Aptitude & General Biotechnology
  1. It binds to the promoter.

  2. Its TBP subunit binds to the region containing the TATA box.

  3. It interacts with TFIIA in the presence of DNA.

  4. It is the RNA polymerase II initiation factor.

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TLS Online TPP Program

#Question id: 18844

#Part-A Aptitude & General Biotechnology

In the extraction of DNA from cell with to remove the high protein content from the cell , before phenol treatment which of the following treatment is done 

TLS Online TPP Program

#Question id: 18845

#Part-A Aptitude & General Biotechnology

The action of EDTA in EDTA-lysozyme treatment in bacterial DNA extraction is

TLS Online TPP Program

#Question id: 18846

#Part-A Aptitude & General Biotechnology

For an application where you require a sample of your target protein at high purity, what would be a good purification strategy? Assume that your starting point is E. coli cells in which the target protein fused to an affinity tag has been over-expressed.

TLS Online TPP Program

#Question id: 18847

#Part-A Aptitude & General Biotechnology

You know that the protein you want to purify from a natural source forms a multimer with multiple sub-units giving a molecular weight in solution much bigger than visualised denatured on SDS-PAGE. There is only a small amount of the target protein in the total protein sample. Which of the following is an appropriate purification strategy?

TLS Online TPP Program

#Question id: 18848

#Part-A Aptitude & General Biotechnology

You find that your protein sample loses activity during storage. What can you do about this?

TLS Online TPP Program

#Question id: 18849

#Part-A Aptitude & General Biotechnology

Which of these techniques is often considered a suitable "polishing" step in a protein purification strategy?