αβ T-cells recognizing MHC plus processed peptide can:

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#Question id: 18955

#Part-A Aptitude & General Biotechnology

(i) (RFLPs), (ii) (RAPDs), (iii) (VNTR) DNA, and (iv) (SSRs) are the examples of

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#Question id: 18957

#Part-A Aptitude & General Biotechnology

A DNA fragment of 5000bp needs to be isolated from E.coli (genome size 4x103kb) genomic library. The minimum number of independent recombinant clones required to represent this fragment in genomic library are

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#Question id: 18958

#Part-A Aptitude & General Biotechnology

The number of clones to represent this fragment in genomic library with a probability of 95% are

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#Question id: 18959

#Part-A Aptitude & General Biotechnology

 Vectors used for construction of genomic libraries, and their capacity is given match them correctly

A- λ EMBL series                                                                                   I - 300kb - 1,400 kb

B - Cosmids                                                                                                 II - 45 kb

C – BACs and YACs (high capacity vectors)                                             III - 20kb

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#Question id: 18960

#Part-A Aptitude & General Biotechnology

cDNA library do not require very large vectors hence which of the following is commonly used in cDNA library

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#Question id: 18961

#Part-A Aptitude & General Biotechnology

When a cDNA library is generated by first removing those mRNA or cDNA sequences that are common to two sources, e.g., two different Cell types, such a library is called