This PCR have mixtures of similar oligonucleotides that are used in a PCR to amplify unknown sequences of DNA, related to a known DNA sequence is

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#Question id: 4357

#Part-A Aptitude & General Biotechnology

Which of the following processes would allow the detection of alternative splicing of transcripts from a given gene?

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#Question id: 13056

#Part-A Aptitude & General Biotechnology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

 Which of the following is true for traditional and real time PCR?

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#Question id: 14984

#Part-B Specialized Branches in Biotechnology

Just beneath the plasma membrane  many protozoa and some algae have a different external, rigid layer of components. The structure is called as

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#Question id: 5316

#Part-A Aptitude & General Biotechnology

Why is sequencing of eukaryotic genomes more difficult than sequencing genomes of bacteria or archaea?

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#Question id: 10567

#Part-B Specialized Branches in Biotechnology

Cell A is having solute potential of -14 and Pressure potential of 3. Cell B is having Solute potential of -16 and Pressure potential of 4. The water will travel from