SYBR Green qPCR method is based on____I_______ with the _____II______ relationship.

TLS Online TPP Program

#Question id: 18962

#Part-A Aptitude & General Biotechnology

1. Presence of oncogenes (iaaM, iaaH and ipt) in T-DNA,

2. Their large size makes the handling procedures during cloning tedious and cumbersome

3. A general lack of unique cloning sites within the T-DNA, which are needed for the insertion of DNA segments to be cloned.

These three problems have been resolved by deleting 

TLS Online TPP Program

#Question id: 18964

#Part-A Aptitude & General Biotechnology

Size of agrobacterium can be reduced by binary vector formation; these binary vectors can be formed by phenomenon of

TLS Online TPP Program

#Question id: 18978

#Part-A Aptitude & General Biotechnology

For expression vector 

TLS Online TPP Program

#Question id: 18979

#Part-A Aptitude & General Biotechnology

For expression vector Strong and Regulatable Promoters are used which have

TLS Online TPP Program

#Question id: 18980

#Part-A Aptitude & General Biotechnology

The most widely used strong regulatable promoters are those

TLS Online TPP Program

#Question id: 18981

#Part-A Aptitude & General Biotechnology

The pL promoter is controlled by the cl repressor protein of bacteriophage A. In practice, a temperature-sensitive mutant of the cl repressor, cl857, is generally used to regulate pL-directed transcription. Cells carrying the temperature-sensitive cl repressor are first grown at 30°C, and 42°C