What will be the major difference between the PCR and replication is?

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#Question id: 755

#Part-B Specialized Branches in Biotechnology

Enzymes that regulate the process of supercoiling termed as;

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#Question id: 13054

#Part-A Aptitude & General Biotechnology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?

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#Question id: 5701

#Part-A Aptitude & General Biotechnology

Cross-over suppressors are production of gametes having cross over products is lethal. Which of the following statement is incorrect?

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#Question id: 19044

#Part-A Aptitude & General Biotechnology

Which of the following techniques would be most useful to identify as well as quantify the presence of a known impurity in a drug substance? 

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#Question id: 14524

#Part-B Specialized Branches in Biotechnology