The first primer in the case of 3’ RACE is ___________

TLS Online TPP Program

#Question id: 13060

#Part-A Aptitude & General Biotechnology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

In blue white screening insertional activation of Beta-D-galactosidase will give rise to

TLS Online TPP Program

#Question id: 406

#Part-B Specialized Branches in Biotechnology

Hydrolysis of 1 M glucose 6-phosphate catalyzed by glucose 6-phosphatase is 99% complete at equilibrium (i.e., only 1% of the substrate remains). Which of the following statements is most nearly correct? (R = 8.315 J/mol·K; T = 298 K)

TLS Online TPP Program

#Question id: 15590

#Part-B Specialized Branches in Biotechnology

Given that Power number is constant; then increasing the RPM of the impeller 3-fold will increase the power consumption due to agitation by:

TLS Online TPP Program

#Question id: 785

#Part-B Specialized Branches in Biotechnology

Several types of very short RNAs repress—or silence—expression of genes with homology to those short RNAs. They are produced from a variety of sources and direct the silencing of genes in the following different ways-

TLS Online TPP Program

#Question id: 1567

#Part-A Aptitude & General Biotechnology

Which of the following cell types are responsible for initiating a secondary immune response?