TLS Online TPP Program

#Question id: 19191


Isothermal amplification is carried out at times. Which of the statement is true?

#Part-A Aptitude & General Biotechnology
  1. The repeated heating and cooling required by PCR is not the reason for limiting how quickly the reaction is carried out
  2. It is carried out typically at 65 degrees
  3. Normal Taq polymerase is used
  4. It requires expensive PCR machines to be carried out
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TLS Online TPP Program

#Question id: 790

#Part-B Specialized Branches in Biotechnology

Presence of a series of 3 G-C pairs in the anticodon arm of initiator fmet tRNA is essential for

TLS Online TPP Program

#Question id: 792

#Part-B Specialized Branches in Biotechnology

Which arm of the tRNA can act as a structural recognition feature?

TLS Online TPP Program

#Question id: 794

#Part-B Specialized Branches in Biotechnology

A single-stranded DNA molecule contains 40 nucleotides with equal amounts of A, C, G, and T. This DNA strand can combine with a complementary DNA strand to form a double-stranded DNA molecule. Which structural feature is found in the single-stranded DNA molecule?

TLS Online TPP Program

#Question id: 795

#Part-B Specialized Branches in Biotechnology

A single-stranded DNA molecule contains 40 nucleotides and has the sequence 5’-(GA)20-3’. When this single strand binds to a complementary DNA strand,

TLS Online TPP Program

#Question id: 797

#Part-B Specialized Branches in Biotechnology

With respect to microRNAs, which, if any, of the following statements, is FALSE?)

A. MicroRNA genes often occur in gene clusters within introns of protein-coding genes and are usually transcribed by RNA polymerase II.

B. Like an mRNA a miRNA is initially produced as a larger precursor RNA that, like the great majority of mRNAs, usually has a 5’ cap and a poly(A) tail.

C. MicroRNAs are produced by germ-line cells only.

D. A miRNA is initially produced as a duplex RNA but in order to work it needs to be converted into a single-strand RNA.

E. MicroRNAs usually work as transcription factors.

TLS Online TPP Program

#Question id: 799

#Part-B Specialized Branches in Biotechnology

The two cleavage reactions required to generate the miRNA from these primary transcripts are mediated by two distinct RNases. A characteristic of both these enzymes is that they recognize and cleave RNAs on the basis of the structure of their substrates rather than their specific sequence. Which of the following statement is INCORRECT regarding the cleavage reactions that produces a mature RNA?