TLS Online TPP Program

#Question id: 19189


Which of the statement is incorrect for anchored PCR?

#Part-A Aptitude & General Biotechnology
  1. Anchored PCR is the modification in which only one piece of the sequence is known and thus one primer
  2. The known sequence is attached to the required region of amplification and then further used as a second priming site
  3. Fragment the sample DNA and ligate it to known sequence
  4. Tails are added enzymatically to the region of known sequence
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TLS Online TPP Program

#Question id: 14119

#Part-B Specialized Branches in Biotechnology

Which of the following is incorrect regarding sequence homology?

TLS Online TPP Program

#Question id: 18733

#Part-A Aptitude & General Biotechnology

Topoisomerase is also an enzyme which is used for carrying out ligation. The correct statement for topoisomerase is?

TLS Online TPP Program

#Question id: 882

#Part-A Aptitude & General Biotechnology

Choose correct statements about thermophilic proteins

I. The proteins found in thermophilic species are much more stable than their mesophilic counterparts.

II. All thermostable proteins have such a high incidence of salt bridges

III. the most important of which are an increased size of the protein’s hydrophobic core, an increased size of the interface between its domains and/or subunits, and a more tightly packed core as evidenced by a reduced surface-to-volume ratio.

IV. Several of these thermostable enzymes have a superabundance of salt bridges on their surfaces.

TLS Online TPP Program

#Question id: 14128

#Part-B Specialized Branches in Biotechnology

Alignment method suitable for aligning closely related sequences? 

TLS Online TPP Program

#Question id: 14164

#Part-B Specialized Branches in Biotechnology

Stoichiometric equations are used to represent growth of microorganisms provided a ' molecular formula' for the cells is available. The molecular formula for biomass is obtained by measuring the amounts of C, N, H, O and other elements in cells. For a particular bacterial strain, the molecular formula was determined to be C4.4H7.30 1.2N0.86 . These bacteria are grown under aerobic conditions with hexadecane (C16H34) as substrate. The reaction equation describing growth is:

                                  

  Assuming 100% conversion, what is the yield of cells from hexadecane in g g-l?