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#Question id: 19186


In another method of quantitative PCR, reporter-quencher set up is used. Which of the statement holds true for this methodology?

#Part-A Aptitude & General Biotechnology
  1. It allows detection of all double stranded molecules
  2. The reporter and quencher are the molecules present on the same probe
  3. The quencher is having a fluorescent group
  4. Fluorescence is observed only when both the groups are present in1- 10 angstrom distance
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#Question id: 3861

#Part-A Aptitude & General Biotechnology

_ are sequences that are removed from primary transcripts, and _ are sequences that remain present in mature mRNA molecules.

TLS Online TPP Program

#Question id: 2302

#Part-A Aptitude & General Biotechnology

In which interval of interphase is DNA duplicated?

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#Question id: 877

#Part-B Specialized Branches in Biotechnology

The sharp transition seen in Figure suggests that

A. Proteins can be denatured by any treatment that disrupts the weak bonds stabilizing tertiary structure, such as heating, or by chemical denaturants such as urea or guanidinium chloride.

B. A comparison of the degree of unfolding as the concentration of denaturant increases reveals a sharp transition from the folded, or native, form to the unfolded, or denatured form, suggesting that only these two conformational states are present to any significant extent.

C. A similar sharp transition is observed if denaturants are removed from unfolded proteins, allowing the proteins to fold.

D. Protein folding and unfolding is an “all or none” process that results from a cooperative transition .

TLS Online TPP Program

#Question id: 14268

#Part-B Specialized Branches in Biotechnology

An activated-sludge waste treatment system is required to reduce the amount of BOD5 from 1000 mg/l to 20 mg/l at the exit. The sedimentation unit concentrates biomass by a factor of 3. Kinetic parameters are µm = 0.2 h-1 , Ks = 80 mg/l, kd = 0.01 h-1 , and Y M X/S = 0.5 g MLVSS/g BOD5. The flow of waste water is 10000 l/h and the size of the treatment basin is 50,000 l. What is the value of the solids residence time?

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#Question id: 10336

#Part-B Specialized Branches in Biotechnology

methemoglobinemia, a disease, What will cause inside the human body?