A DNA fragment of 5000bp needs to be isolated from E.coli (genome size 4x103kb) genomic library. The minimum number of independent recombinant clones required to represent this fragment in genomic library are

TLS Online TPP Program

#Question id: 18753

#Part-A Aptitude & General Biotechnology

For mutagenesis without PCR, which of the following can be used as a template?

TLS Online TPP Program

#Question id: 18754

#Part-A Aptitude & General Biotechnology

An oligonucleotide is synthesized which contains the mutation and the rest is _______ to the template DNA.

TLS Online TPP Program

#Question id: 18755

#Part-A Aptitude & General Biotechnology

Once the double stranded molecule with the mutation is introduced into E. coli for replication, how many types of molecules are produced?

TLS Online TPP Program

#Question id: 18756

#Part-A Aptitude & General Biotechnology

If mixed oligonucleotides are used, it is regarded as ______________

TLS Online TPP Program

#Question id: 18757

#Part-A Aptitude & General Biotechnology

which phage is used in oligonucleotide directed mutagenesis?

TLS Online TPP Program

#Question id: 18758

#Part-A Aptitude & General Biotechnology

Which polymerase is used in PCR based mutagenesis?