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TLS Online TPP Program

#Question id: 18852

What is the starting point for selection of a suitable IEX matrix for purification of a recombinant protein?

#Part-A Aptitude & General Biotechnology
  1. Prediction of isoelectric point (pI) from the amino acid sequence
  2. Test protein binding to an IEX matrix at a range of pHs and salt concentrations
  3. Test protein binding to a selection of anion and cation exchange matrices
  4. Pass your sample through a preparative column and elute with a salt gradient
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TLS Online TPP Program

#Question id: 3658

#Part-A Aptitude & General Biotechnology

In E. coli, the UvrABC endonuclease removes _____________and other bulky lesions by recognizing the distortion these produce in the double helix.
TLS Online TPP Program

#Question id: 15377

#Part-B Specialized Branches in Biotechnology

 Find the Laplace transform of et sin(t)
TLS Online TPP Program

#Question id: 2071

#Part-A Aptitude & General Biotechnology

Diffusion of ions across membranes through specific ion channels is driven by:
TLS Online TPP Program

#Question id: 11538

#Part-B Specialized Branches in Biotechnology

Prostaglandins are local regulators whose chemical structure is derived from ________.
TLS Online TPP Program

#Question id: 11193

#Part-B Specialized Branches in Biotechnology

Conduction and refractory periods (states) are typical of ________.