TLS Online TPP Program

#Question id: 18852


What is the starting point for selection of a suitable IEX matrix for purification of a recombinant protein?

#Part-A Aptitude & General Biotechnology
  1. Prediction of isoelectric point (pI) from the amino acid sequence
  2. Test protein binding to an IEX matrix at a range of pHs and salt concentrations
  3. Test protein binding to a selection of anion and cation exchange matrices
  4. Pass your sample through a preparative column and elute with a salt gradient
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TLS Online TPP Program

#Question id: 21726

#Part-B Specialized Branches in Biotechnology

For a first order chemical reaction, the rate constant

TLS Online TPP Program

#Question id: 21727

#Part-B Specialized Branches in Biotechnology

In the reaction H2O2(aq) → H2O(l) + ½ O2(g), the initial concentration of H2O2 is 0.2546 M, and the initial rate of reaction is 9.32×10–4 M s–1. What will be [H2O2] at t = 35 s?

TLS Online TPP Program

#Question id: 21728

#Part-B Specialized Branches in Biotechnology

In the reaction A → products, we find that when [A] has fallen to half of its initial value, the reaction proceeds at the same rate as its initial rate. Is the reaction zero order, first order, or second order?

TLS Online TPP Program

#Question id: 21729

#Part-B Specialized Branches in Biotechnology

In the reaction, A → products, with the initial concentration [A]0 = 1.512 M, [A] is found to be 1.496 M at t = 30 s. With the initial concentration [A]0 = 2.584 M, [A] is found to be 2.552 M at t = 1 min. What is the order of this reaction?

TLS Online TPP Program

#Question id: 21731

#Part-B Specialized Branches in Biotechnology

The smog constituent peroxyacetyl nitrate (PAN) dissociates into peroxyacetyl radicals and NO2(g) in a first order reaction with a half-life of 32 min. 
If the initial concentration of PAN in an air sample is 2.7×1015 molecules/L, what will be the concentration 2.24 h later?

TLS Online TPP Program

#Question id: 21733

#Part-B Specialized Branches in Biotechnology

A rate law __________.