TLS Online TPP Program

#Question id: 18851


Nickel-NTA (Ni-NTA) chromatography is a popular affinity chromatography method for the purification of histidine-tagged proteins. However, SDS-PAGE of the eluted protein can show bands in addition to your target protein. How might you improve this purification step?

#Part-A Aptitude & General Biotechnology
  1. Add a low concentration of imidazole to the binding buffer
  2. Gradient elution with increasing imidazole concentration on a chromatography machine
  3. Include protease inhibitors during purification
  4. All of the above
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TLS Online TPP Program

#Question id: 11290

#Part-B Specialized Branches in Biotechnology

Which of the following statement are incorrect?

TLS Online TPP Program

#Question id: 11290

#Part-B Specialized Branches in Biotechnology

Which of the following statement are incorrect?

TLS Online TPP Program

#Question id: 11291

#Part-B Specialized Branches in Biotechnology

A small lake has three trophic levels- phytoplankton (autotrophs), Zooplankton (herbivore) and planktivorous fish (primary carnivore). In this lake, addition of nutrients is allowed to study the 'bottom up' effects". What is the expected biomass of phytoplankton and zooplankton tropic levels?

TLS Online TPP Program

#Question id: 11291

#Part-B Specialized Branches in Biotechnology

A small lake has three trophic levels- phytoplankton (autotrophs), Zooplankton (herbivore) and planktivorous fish (primary carnivore). In this lake, addition of nutrients is allowed to study the 'bottom up' effects". What is the expected biomass of phytoplankton and zooplankton tropic levels?

TLS Online TPP Program

#Question id: 11291

#Part-A Aptitude & General Biotechnology

A small lake has three trophic levels- phytoplankton (autotrophs), Zooplankton (herbivore) and planktivorous fish (primary carnivore). In this lake, addition of nutrients is allowed to study the 'bottom up' effects". What is the expected biomass of phytoplankton and zooplankton tropic levels?

TLS Online TPP Program

#Question id: 11292

#Part-B Specialized Branches in Biotechnology

In an pond ecosystem the _____ is always greater than the _____ .

(a) number of primary producers . . . number of secondary consumers

(b) biomass of secondary consumers . . . biomass of primary consumer

(c) assimilation efficiency of primary consumers . . . assimilation efficiency of secondary consumers

(d) biomass of primary producers . . . biomass of primary consumers