You know that the protein you want to purify from a natural source forms a multimer with multiple sub-units giving a molecular weight in solution much bigger than visualised denatured on SDS-PAGE. There is only a small amount of the target protein in the total protein sample. Which of the following is an appropriate purification strategy?

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#Question id: 13058

#Part-A Aptitude & General Biotechnology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following true about real time PCR?

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#Question id: 11059

#Part-B Specialized Branches in Biotechnology

A healthy, 45-year-old man is reading the newspaper. Which of the following muscles are used for quiet breathing?

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#Question id: 14213

#Part-B Specialized Branches in Biotechnology

Which phase has the condition of specific growth rate “μ = 0”?

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#Question id: 19059

#Part-A Aptitude & General Biotechnology

Which of the following is not used for detection in GC?

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#Question id: 685

#Part-A Aptitude & General Biotechnology

A protein has a molecular weight of 5600 daltons; its subunits are about 1960 daltons. There are ________ protein chains per oligomer.