TLS Online TPP Program

#Question id: 18834


A researcher is trying to alter five successive base pairs in the middle of a PCR amplified DNA fragments. Which technique is the most recommended for performing such experiment? 

#Part-A Aptitude & General Biotechnology
  1. DNA ligation
  2. Electrophoretic mobility shift assay (EMSA)
  3. PCR mutagenesis
  4. Restriction enzyme digestion
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TLS Online TPP Program

#Question id: 14259

#Part-B Specialized Branches in Biotechnology

C. roseus cells immobilized in Ca-alginate beads of diameter 0.5 mm are used for production of indole alkaloids (IA) in a fluidized-bed bioreactor. The rate limiting nutrient is glucose and no intraparticle diffusion limitations exist. Use the following data: Flow rate of the feed: Q = 1 l/h, Glucose in the feed: So = 30 g/l, Plant Cell Concentration: X = 6 g/l reac. The rate constant for IA formation: k = 5 d-1 (g/l)^-1 Ks = 0.4 g/l, Column diameter: Do = 0.15 m. Growth is negligible and Monod kinetics is valid. For 95% glucose conversion determine volume. 

TLS Online TPP Program

#Question id: 18668

#Part-A Aptitude & General Biotechnology

Enzymes and antibody can easily be detected in samples subjected to electrophoresis on cellulose acetate, by using the 

TLS Online TPP Program

#Question id: 2953

#Part-A Aptitude & General Biotechnology

If a cell in the G1 phase is fused with a cell in the S phase, then

TLS Online TPP Program

#Question id: 20601

#Part-B Specialized Branches in Biotechnology

The difference between the set points and the measured signal is known as ______________?

TLS Online TPP Program

#Question id: 21006

#Part-B Specialized Branches in Biotechnology

Match the group 1 with appropriate description of group 2.
Group 1                                                  group 2
(P) Pectinases                                    (1) Meat tenderizer
(Q) Lipases                                         (2) clarification of fruit juice
(R) Amylases                                      (3) cheese and butter industry
(S) Trypsin                                           (4) starch-liquefying enzyme