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#Question id: 18748


Which of the following is not a condition for PCR in single cycle?

#Part-A Aptitude & General Biotechnology
  1. Initial Denaturation carried out for 0.5 minutes at 94 degrees
  2. Primer annealing carried out for 1.5 minutes at 55 degrees
  3. Initial Denaturation carried out for 3 minutes at 55 degrees
  4. DNA Extension  at 72 degrees for 1 minutes

Answer:- Option(s): 3


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