When a protein exists in mono-, di- and tri-phosphorylated forms. The difference of a couple of phosphate groups has no significant effect on the overall relative molecular mass of the protein, hence a single band on SDS gels, but the small charge difference introduced on each molecule can be detected by

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#Question id: 18778

#Part-A Aptitude & General Biotechnology

which of the following technique provides an unusually powerful technology for the quantitative analysis and sorting of cell populations labelled with one or more fluorescent antibodies.

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#Question id: 18779

#Part-A Aptitude & General Biotechnology

Flow-cytometric analysis for the detection and measurement of subpopulations of leukocytes, including those of leukaemia, is usually performed using monoclonal antibodies. Why is this the case?

Choose whether each of the following statements is true 

a) Indirect immunofluorescence is a more sensitive technique than direct immunofluorescence

b) Most antigens induce a polyclonal response. 

c) For precipitation to occur, both antigen and antibody must be multivalent.

d) ELISA tests using chemiluminescence are more sensitive than chromogenic ones and precipitation tests are more sensitive than agglutination tests

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#Question id: 18780

#Part-A Aptitude & General Biotechnology

Which of the following does not participate in the formation of antigen-antibody complexes?

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#Question id: 18781

#Part-A Aptitude & General Biotechnology

In which of the following case a large lattice is formed?

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#Question id: 18782

#Part-A Aptitude & General Biotechnology

The concentration of a small amount antibodies 2 μg antibody/ml can be detected

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#Question id: 18783

#Part-A Aptitude & General Biotechnology

Sensitivity of various immunoassays are given match them correctly

Assay	Sensitivity∗ (g antibody/ml)
a) Mancini radial immunodiffusion	i) 2
b) Ouchterlony double immunodiffusion	ii) 20–200
c) Immunoelectrophoresis	iii) 20–200
d) Rocket electrophoresis	iv) 10–50