TLS Online TPP Program

#Question id: 14119


Which of the following is incorrect regarding sequence homology?

#Part-B Specialized Branches in Biotechnology
  1. It is an important concept in sequence analysis
  2. When two sequences are descended from a common evolutionary origin, they are said to share homology
  3. Two sequences can homologous relationship even if have do not have common origin
  4. When two sequences are descended from a common evolutionary origin, they are said to have a homologous relationship
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TLS Online TPP Program

#Question id: 13077

#Part-A Aptitude & General Biotechnology

The DNA probes for RFLP may NOT be obtained from

TLS Online TPP Program

#Question id: 13078

#Part-A Aptitude & General Biotechnology

RFLPs have several unique advantages except this one;

TLS Online TPP Program

#Question id: 13079

#Part-A Aptitude & General Biotechnology

Name of that sophisticated equipment which used to the oligonucleotide, that serve as a primer in RAPDs is usually obtained by in vitro DNA synthesis,

TLS Online TPP Program

#Question id: 13080

#Part-A Aptitude & General Biotechnology

In RAPDs amplification will take place only of those regions of the genome that have the 

TLS Online TPP Program

#Question id: 13081

#Part-A Aptitude & General Biotechnology

Correct statements about RAPD’s
A. RAPD polymorphism is detected by using oligonucleotides usually more than 10 bases long of random sequences as primers in a reaction.
B. In a strain which has in genomic DNA sequences complementary to the primer oligonucleotide, PCR products will be detected in the gel,
C. Typical RAPD markers show limited variation between parents, especially in naturally inbreeding species.
D. RAPDs are more sensitive than RFLPs to experimental conditions making them more difficult to be consistent and reproducible.

TLS Online TPP Program

#Question id: 13082

#Part-A Aptitude & General Biotechnology

Statement: AFLP shares some features of both RFLP and RAPD analyses.
Explanations: I. It uses restriction enzyme-digested genomic DNA as template for PCR amplification using primers that contain the restriction enzyme recognition sites plus a number of, usually 2-3, arbitrary nucleotides.
II. AFLPs are faster, less labour intensive and provide more information than RFLPs, and they are highly reproducible, which is a great advantage over RAPDs.