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TLS Online TPP Program

#Question id: 801

Identify the type of DNA from the structures given below and match the correct form with its characteristics described.

#Part-B Specialized Branches in Biotechnology

  1. (ii) – The more compact A form of DNA has 11 bp per turn and shows a large tilt of the base pairs with respect to the helix axis.

  2. (i) – The B form, which is observed at high humidity, most closely corresponds to the average structure of DNA under physiological conditions.

  3. (iii) – Z DNA is a left-handed helix and has a zigzag (hence “Z”) appearance and has an anti-conformation of glycosyl bond.

  4. (i) – The A form which is observed under low humidity has a structure similar to the structure that RNA adopts when double-helical.

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TLS Online TPP Program

#Question id: 18776

#Part-A Aptitude & General Biotechnology

In Western blotting, a protein mixture is separated by electrophoresis; then the protein bands are electrophoretically transferred onto nitrocellulose and identified with 
TLS Online TPP Program

#Question id: 18777

#Part-A Aptitude & General Biotechnology

which of the following technique using antibodies labelled with fluorescent molecules can be used to visualize antigen on or within cells.
TLS Online TPP Program

#Question id: 18778

#Part-A Aptitude & General Biotechnology

which of the following technique provides an unusually powerful technology for the quantitative analysis and sorting of cell populations labelled with one or more fluorescent antibodies.
TLS Online TPP Program

#Question id: 18779

#Part-A Aptitude & General Biotechnology

Flow-cytometric analysis for the detection and measurement of subpopulations of leukocytes, including those of leukaemia, is usually performed using monoclonal antibodies. Why is this the case?
Choose whether each of the following statements is true 
a) Indirect immunofluorescence is a more sensitive technique than direct immunofluorescence
b) Most antigens induce a polyclonal response. 
c) For precipitation to occur, both antigen and antibody must be multivalent.
d) ELISA tests using chemiluminescence are more sensitive than chromogenic ones and precipitation tests are more sensitive than agglutination tests
TLS Online TPP Program

#Question id: 18780

#Part-A Aptitude & General Biotechnology

Which of the following does not participate in the formation of antigen-antibody complexes?
TLS Online TPP Program

#Question id: 18781

#Part-A Aptitude & General Biotechnology

In which of the following case a large lattice is formed?