Alexander Varshavsky discovered, the half-lives of many cytoplasmic proteins vary with the identities of their N-terminal residues via the so-called N-end rule:-

A. Destabilizing N-terminal residues Asp, Arg, Leu, Lys, and Phe have half-lives of	i. Only 2 to 3 minutes,
B. Stabilizing N-terminal residues Ala, Gly, Met, Ser, Thr, and Val have half-lives of	ii. >10 hours in prokaryotes iii. >20 hours in eukaryotes.

TLS Online TPP Program

#Question id: 19789

#Part-A Aptitude & General Biotechnology

How to determine the size of PCR product?

TLS Online TPP Program

#Question id: 19789

#Part-B Specialized Branches in Biotechnology

How to determine the size of PCR product?

TLS Online TPP Program

#Question id: 19790

#Part-A Aptitude & General Biotechnology

What will be the major difference between the PCR and replication is?

TLS Online TPP Program

#Question id: 19790

#Part-A Aptitude & General Biotechnology

What will be the major difference between the PCR and replication is?

TLS Online TPP Program

#Question id: 19791

#Part-A Aptitude & General Biotechnology

Which of the following statements about polymerase chain reaction is correct?

a) PCR denaturation temperature calculated by using Y = X (1+E)n

b) Size of the primer negatively affects the efficiency of PCR

c) Standard PCR doesn’t efficiently amplify sequence much longer than about 5kb.

d) Primer annealing of PCR at 550C for 1.5 min.

e) Time taken for each cycle of PCR considerably longer than 3 min.

TLS Online TPP Program

#Question id: 19791

#Part-A Aptitude & General Biotechnology

Which of the following statements about polymerase chain reaction is correct?

a) PCR denaturation temperature calculated by using Y = X (1+E)n

b) Size of the primer negatively affects the efficiency of PCR

c) Standard PCR doesn’t efficiently amplify sequence much longer than about 5kb.

d) Primer annealing of PCR at 550C for 1.5 min.

e) Time taken for each cycle of PCR considerably longer than 3 min.