The folding pathways of some proteins require two enzymes that catalyze isomerization reactions such as,

a) Peptide prolyl isomerase (PPI)  catalyzes the elimination of folding intermediates with inappropriate disulfide cross-links

b) Protein disulfide isomerase (PDI) enzyme that catalyzes the interchange, or shuffling, of disulfide bonds until the bonds of the native conformation are formed

c) Peptide prolyl cis-trans isomerase (PPI) catalyzes the interconversion of the cis and trans isomers of Proline residue peptide bonds

d) Peptide prolyl isomerase (PPI) can be a fast step in the folding of proteins that contain some Pro peptide bonds in the cis conformation

Which of the following combinations is correct about protein folding pathways?

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#Question id: 18704

#Part-A Aptitude & General Biotechnology

If the weight of 1 mol bp is 660g What will the no. of moles in human genome having 3 x 10⁹ bp

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#Question id: 18705

#Part-A Aptitude & General Biotechnology

What will weight in picogram in human genome 

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#Question id: 18706

#Part-A Aptitude & General Biotechnology

Sources of thermostable DNA polymerases with proofreading (3′–5′ exonuclease) activity are given match them correctly

DNA polymerase                          Source

a. Tma                                      i. Thermotoga maritima

b. Deep vent tm                      ii. Pyrococcus furiosus

c. Tli                                      iii. Thermococcus litoralis

d. Pfu                                      iv.  Pyrococcus sp

e. Pwo                                      v. Pyrococcus woesi

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#Question id: 18707

#Part-A Aptitude & General Biotechnology

primer dimers are formed when 

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#Question id: 18708

#Part-A Aptitude & General Biotechnology

PCR can be analysed amplification, Anneleaning, and extension and analysis is called

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#Question id: 18709

#Part-A Aptitude & General Biotechnology

qPCR can be done by 

i) cyanin dye or SYBR green – to measure amplification

ii) Taqman assay – measure extension

iii) molecular beacons - denaturation