Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  Determine the volume of the packed bed for 95% glucose conversion. Bed diameter is D0 = 0.2 m. Neglect the growth of the hybridomas and assume first order kinetics. 

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#Question id: 14282

#Part-B Specialized Branches in Biotechnology

The oxygen transfer rate in an aerobic fermentation process does not depend on the

TLS Online TPP Program

#Question id: 14282

#Part-A Aptitude & General Biotechnology

The oxygen transfer rate in an aerobic fermentation process does not depend on the

TLS Online TPP Program

#Question id: 14283

#Part-B Specialized Branches in Biotechnology

Aeration ina fermenter is expressed as VVM. What will be the VVM if air is sparged at 2000 L/min with a working volume 10 m^3? 

TLS Online TPP Program

#Question id: 14283

#Part-A Aptitude & General Biotechnology

Aeration ina fermenter is expressed as VVM. What will be the VVM if air is sparged at 2000 L/min with a working volume 10 m^3? 

TLS Online TPP Program

#Question id: 14318

#Part-B Specialized Branches in Biotechnology

system for manufacture of single-cell protein is Methylophilus methylotrophus. This organism is produced aerobically from methanol with ammonia as nitrogen source. The molecular formula for the biomass is CH _1.680_0.36N_0.22; these cells contain 6% ash. If the actual yield of biomass from methanol is 42% the thermodynamic maximum, what is the oxygen demand?

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#Question id: 14331

#Part-B Specialized Branches in Biotechnology

To ensure turbulent conditions and minimum mixing time during agitation with a turbine impeller, the Reynolds number must be at least 10⁴. A stirred laboratory-scale fermenter with a turbine impeller 5 cm in diameter is operated at 800 rpm. If the density of broth being stirred is close to that of water, what is the upper limit for viscosity of the suspension if adequate mixing is to be maintained?