Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  Determine the volume of the packed bed for 95% glucose conversion. Bed diameter is D0 = 0.2 m. Neglect the growth of the hybridomas and assume first order kinetics. 

TLS Online TPP Program

#Question id: 13052

#Part-B Specialized Branches in Biotechnology

What would be the effect on the PCR reaction if any of the following circumstances arose: 1) there are no primers in the reaction, 2) there are no dNTPs in the reaction, 3) there is no Taq polymerase in the reaction?

TLS Online TPP Program

#Question id: 11869

#Part-A Aptitude & General Biotechnology

Find the least number which when divided separately by 15, 20, 36 and 48 leaves 3 as remainder in each case.

TLS Online TPP Program

#Question id: 13042

#Part-A Aptitude & General Biotechnology

For analyzing DNA sequences, two techniques have been developed, one is _________ method frequently termed_________, involving the enzymatic synthesis of DNA and stopping the synthesis at specific nucleotides. While another technique, the __________ method of DNA sequencing developed by ___________ is often used for sequencing small fragments of DNA.

TLS Online TPP Program

#Question id: 9276

#Part-B Specialized Branches in Biotechnology

The carotenoid b-carotene, whose structure and absorption spectrum at,

TLS Online TPP Program

#Question id: 11460

#Part-A Aptitude & General Biotechnology

The round window ________.

I) dampens fluid vibrations

II) collects sound pressure waves

III) detects the frequency of sounds