Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  Determine the volume of the packed bed for 95% glucose conversion. Bed diameter is D0 = 0.2 m. Neglect the growth of the hybridomas and assume first order kinetics. 

#Question id: 732

#Part-B Specialized Branches in Biotechnology

Ramachandran plots can help increase the accuracy of models derived from x-ray crystallography data. The plot below was created by measuring phi, and psi for each amino acid residue in a 2.2 A resolution crystal structure. In the plot, which excludes Gly and Pro residues, selected residues (dots) are numbered 1 through 5.

Which of the following statements can be correctly stated for this plot in reference to selected residues?

A. Residues 1 and 3 are in either B sheets

B. residue 2 is in a right-handed a helix, and residue 4 is in a left-handed a helix.

C. If residue 5 were Gly, it might be in an acceptable position in the plot

#Question id: 734

#Part-B Specialized Branches in Biotechnology

Which of the following statements is true for two different tripeptides consisting of either glycine or proline?

#Question id: 736

#Part-B Specialized Branches in Biotechnology

The most important contribution to the stability of a protein’s conformation appears to be the:

#Question id: 738

#Part-B Specialized Branches in Biotechnology

Which of the following is correct for peptide bonds

A) C and N are shorter than typical C-N bonds.

B) C and N are longer than typical C-N bonds.

C) C and O are longer than typical C=O bonds.

D) C and O are shorter than typical C=O bonds.

#Question id: 740

#Part-B Specialized Branches in Biotechnology

The conformation of the backbone of a polypeptide is described completely by the angle(s) of rotation about which bond(s)?

#Question id: 742

#Part-B Specialized Branches in Biotechnology

Match the following