TLS Online TPP Program

#Question id: 14254


Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  Determine the height of the packed bed for 95% glucose conversion. Bed diameter is D0 = 0.2 m. Neglect the growth of the hybridomas and assume first order kinetics. 

#Part-B Specialized Branches in Biotechnology
  1. 0.55m
  2. 0.95m   
  3. 1.25m
  4. 0.75m  
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TLS Online TPP Program

#Question id: 2982

#Part-B Specialized Branches in Biotechnology

Match the following proteins (Column I) with their functions (Column II) during cell cycle.

Column I

Column II

A. Cdc25C phosphatase

i. Activates vertebrate S phase CDKs

B. INK4

ii. Activation of Mitotic CDKs

C. Cdc25A phosphatase

iii. Binds and inhibits G1 CDKs

D. APC/CCdc20

iv. Degradation of securin

TLS Online TPP Program

#Question id: 2987

#Part-B Specialized Branches in Biotechnology

Following statements are regarding the goals of gap phases.

(i) to prepare for the next phase of the cell cycle.

(ii) to check that the previous phase of the cell cycle has been completed appropriately.

(iii) to inhibits CDKs activity.

(iv) to enhance servillance mechanisms.

Which of the following is correct?

TLS Online TPP Program

#Question id: 2988

#Part-A Aptitude & General Biotechnology

Use the figure to answer the question below. 

If the cell whose nuclear material is shown in the figure continues toward completion of mitosis, which of the following events would occur next?

TLS Online TPP Program

#Question id: 2994

#Part-B Specialized Branches in Biotechnology

If a cell has completed meiosis I and the first cytokinesis, and is just beginning meiosis II, which of the following is an appropriate description of its genetic contents?

TLS Online TPP Program

#Question id: 2995

#Part-A Aptitude & General Biotechnology

 Which of the following incorrect pair?

TLS Online TPP Program

#Question id: 2996

#Part-A Aptitude & General Biotechnology

Cell death approximately equal to cell division is found in