Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  Determine the height of the packed bed for 95% glucose conversion. Bed diameter is D0 = 0.2 m. Neglect the growth of the hybridomas and assume first order kinetics. 

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#Question id: 18817

#Part-A Aptitude & General Biotechnology

The PCR technique widely used in bacterial genomic studies for screening of plasmid inserts is

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#Question id: 12882

#Part-A Aptitude & General Biotechnology

The radii of the base of two cylinders are in the ratio 3 : 5 and their heights in the ratio 2 : 3. The ratio of their curved surface will be :

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#Question id: 11096

#Part-B Specialized Branches in Biotechnology

In which structure does diffusion of oxygen to capillaries not play a major role?

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#Question id: 12678

#Part-A Aptitude & General Biotechnology

The length of the diagonal of a square is ‘a’ cm. Which of the following represents the area of the square (in sq. cm.) ?

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#Question id: 709

#Part-A Aptitude & General Biotechnology

A sequence of amino acids in a certain protein is found to be -Ser-Gly-Pro-Gly-.  The sequence is most probably part of a(n):