Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  If Yp/s is 4 mg Mab/g glu, determine the effluent Mab concentration of the system?

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#Question id: 18958

#Part-A Aptitude & General Biotechnology

The number of clones to represent this fragment in genomic library with a probability of 95% are

TLS Online TPP Program

#Question id: 18959

#Part-A Aptitude & General Biotechnology

 Vectors used for construction of genomic libraries, and their capacity is given match them correctly

A- λ EMBL series	I - 300kb - 1,400 kb
B - Cosmids	II - 45 kb
C - BACs and YACs (high capacity vectors)	III - 20kb

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#Question id: 18960

#Part-A Aptitude & General Biotechnology

cDNA library do not require very large vectors hence which of the following is commonly used in cDNA library

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#Question id: 18961

#Part-A Aptitude & General Biotechnology

When a cDNA library is generated by first removing those mRNA or cDNA sequences that are common to two sources, e.g., two different Cell types, such a library is called 

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#Question id: 18962

#Part-A Aptitude & General Biotechnology

1. Presence of oncogenes (iaaM, iaaH and ipt) in T-DNA,

2. Their large size makes the handling procedures during cloning tedious and cumbersome

3. A general lack of unique cloning sites within the T-DNA, which are needed for the insertion of DNA segments to be cloned.

These three problems have been resolved by deleting 

TLS Online TPP Program

#Question id: 18964

#Part-A Aptitude & General Biotechnology

Size of agrobacterium can be reduced by binary vector formation; these binary vectors can be formed by phenomenon of