Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V0 = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S0 = 300 g/l and X0 = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h-1 , KS = 0.5 g/l, and YX/S = 0.3 g dw/g glucose. If qP = 0.05 g product/g cells h and P₀ = 0.1 g/l, determine the product concentration in the vessel at t = 10 h

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#Question id: 18677

#Part-A Aptitude & General Biotechnology

Point mutations in DNA, such as occur in a range of human diseases, can be identified by 

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#Question id: 18678

#Part-A Aptitude & General Biotechnology

which of the following technique can be analysed of PCR products from HIV-I allowed the identification of between 200 000 and 500 000 viral particles per cubic centimetre of serum.

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#Question id: 18679

#Part-A Aptitude & General Biotechnology

Chiral compounds can be resolved using

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#Question id: 18680

#Part-A Aptitude & General Biotechnology

DNA from Salmonella species, when digested with the restriction enzyme Xba I, gives around 15 fragments ranging from 25 kb to 680 kb This pattern of fragments, or ‘fingerprint’, is unique to that strain. If the same fingerprint is found from bacteria from other infected people, then it can be assumed that they were all infected from a common source. Thus, by comparing their eating habits, food sources, etc. the source of the infection can be traced to a restaurant, food item. The fingerprints are created and analysed by

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#Question id: 18681

#Part-A Aptitude & General Biotechnology

Highly heterogeneous mixtures of proteins such as cell lysates or tissue extracts should be completely fractionated into individual proteins so as to obtain an overall picture of the protein composition and to enable location of individual proteins. The highest resolution is obtained 

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#Question id: 18682

#Part-A Aptitude & General Biotechnology

Which technique is shown in given representation