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TLS Online TPP Program

#Question id: 21006

Match the group 1 with appropriate description of group 2.

Group 1Group 2
(P) Pectinases(1) Meat tenderizer
(Q) Lipases(2) clarification of fruit juice
(R) Amylases(3) cheese and butter industry
(S) Trypsin(4) starch-liquefying enzyme

#Part-B Specialized Branches in Biotechnology
  1. P-3, Q-4, R-2, S-1
  2. P-2, Q-3, R-4, S-1 
  3. P-3, Q-2, R-1, S-4 
  4. P-1, Q-2, R-4, S-3 
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TLS Online TPP Program

#Question id: 14217

#Part-B Specialized Branches in Biotechnology

In a microbial system how true yields are related to apparent growth yield? 
TLS Online TPP Program

#Question id: 14218

#Part-B Specialized Branches in Biotechnology

In CSTR system at steady rate  
TLS Online TPP Program

#Question id: 14218

#Part-A Aptitude & General Biotechnology

In CSTR system at steady rate  
TLS Online TPP Program

#Question id: 14221

#Part-B Specialized Branches in Biotechnology

E. coli have a maximum respiration rate, qO2max, of about 240-mg O2/g-dry wt-h. It is desired to achieve a cell mass of 20 g dry wt/l. The kLa is 120 h-1 in a 1000-l reactor (800 l working volume). A gas stream enriched in oxygen is used (i.e., 80% O2) which gives a value of C* = 28 mg/L. If oxygen becomes limiting, growth and respiration slow; for example,

                                     
   where CL is the dissolved oxygen concentration in the fermenter. What is CL when the cell mass is  at 20 g/l?
TLS Online TPP Program

#Question id: 14237

#Part-B Specialized Branches in Biotechnology

A genetically-engineered strain of yeast is cultured in a bioreactor at 30⁰C for production of heterologous protein. The oxygen requirement is 80 mmol 1-1 h-I; the critical oxygen concentration is 0.004 mM. The solubility of oxygen in the fermentation broth is estimated to be 10% lower than in water due to solute effects. What is the minimum mass-transfer coefficient necessary to sustain this culture if the reactor is sparged with air at approximately 1 atm pressure ?         
TLS Online TPP Program

#Question id: 14238

#Part-B Specialized Branches in Biotechnology

A genetically-engineered strain of yeast is cultured in a bioreactor at 30⁰C for production of heterologous protein. The oxygen requirement is 80 mmol 1-1 h-I; the critical oxygen concentration is 0.004 mM. The solubility of oxygen in the fermentation broth is estimated to be 10% lower than in water due to solute effects. What mass-transfer coefficient is required if pure oxygen is used instead of air?