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TLS Online TPP Program

#Question id: 574

Which of the following statements is FALSE?

#Part-B Specialized Branches in Biotechnology

  1. Enzymes make reactions 103 to 1020 times faster.

  2. Enzymes lower the amount of energy needed for a reaction.

  3. Enzymes are chemically unchanged during the actual catalytic process.

  4. Enzymes speed up the attainment of a reaction equilibrium.

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TLS Online TPP Program

#Question id: 13074

#Part-A Aptitude & General Biotechnology

Type of probe used in RFLP;
TLS Online TPP Program

#Question id: 13075

#Part-A Aptitude & General Biotechnology

Radioisotopes must be used in RFLP and RAPD markers
TLS Online TPP Program

#Question id: 13076

#Part-A Aptitude & General Biotechnology

Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.
TLS Online TPP Program

#Question id: 13077

#Part-A Aptitude & General Biotechnology

The DNA probes for RFLP may NOT be obtained from
TLS Online TPP Program

#Question id: 13078

#Part-A Aptitude & General Biotechnology

RFLPs have several unique advantages except this one;
TLS Online TPP Program

#Question id: 13079

#Part-A Aptitude & General Biotechnology

Name of that sophisticated equipment which used to the oligonucleotide, that serve as a primer in RAPDs is usually obtained by in vitro DNA synthesis,