TLS Online TPP Program

#Question id: 10280


The enzyme of glycolytic pathway that forms fructose 1,6-bisphosphate is called phosphofructokinase-1 (PFK-1), which statements can’t distinguish it from a second enzyme (PFK-2) Phosphofructokinase-2.

#Part-B Specialized Branches in Biotechnology
  1. PFK-2 that catalyzes the formation of fructose 2,6-bisphosphate from fructose 6-phosphate rather than fructose 1,6-bisphosphate in a separate pathway
  2. PFK-2 activity is regulated by phosphorylation while the PFK-1 activity is regulated by high conc of ATP and NADH etc
  3. more important regulator of plant glycolysis is the cytosolic concentration of PEP, which is an Activator of the plant ATP-dependent PFK-1 and inhibitor of PFK-2
  4. fructose 2,6-bisphosphate activates PFK-1 by increasing its apparent affinity for fructose 6-phosphate
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TLS Online TPP Program

#Question id: 18778

#Part-A Aptitude & General Biotechnology

which of the following technique provides an unusually powerful technology for the quantitative analysis and sorting of cell populations labelled with one or more fluorescent antibodies.

TLS Online TPP Program

#Question id: 18779

#Part-A Aptitude & General Biotechnology

Flow-cytometric analysis for the detection and measurement of subpopulations of leukocytes, including those of leukaemia, is usually performed using monoclonal antibodies. Why is this the case?
Choose whether each of the following statements is true 
a) Indirect immunofluorescence is a more sensitive technique than direct immunofluorescence
b) Most antigens induce a polyclonal response. 
c) For precipitation to occur, both antigen and antibody must be multivalent.
d) ELISA tests using chemiluminescence are more sensitive than chromogenic ones and precipitation tests are more sensitive than agglutination tests

TLS Online TPP Program

#Question id: 18780

#Part-A Aptitude & General Biotechnology

Which of the following does not participate in the formation of antigen-antibody complexes?

TLS Online TPP Program

#Question id: 18781

#Part-A Aptitude & General Biotechnology

In which of the following case a large lattice is formed?

TLS Online TPP Program

#Question id: 18782

#Part-A Aptitude & General Biotechnology

The concentration of a small amount antibodies 2 μg antibody/ml can be detected

TLS Online TPP Program

#Question id: 18783

#Part-A Aptitude & General Biotechnology

Sensitivity of various immunoassays are given match them correctly

AssaySensitivity* (μg antibody/ml)
a) Mancini radial immunodiffusioni) 2
b) Ouchterlony double immunodiffusionii) 20–200
c) Immunoelectrophoresisiii) 20–200
d) Rocket electrophoresisiv) 10–50