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TLS Online TPP Program

#Question id: 19888

There are some typical enzyme used in the manipulation of nucleic acid;

                 

                 ENZYMES

 

USE IN NUCLEIC ACID MANIPULATION

 

A) klenow

 

i) DNA-dependent RNA polymerase

 

B) T4 DNA polymerase

 

ii) Single-strand specific nuclease

 

C) Taq DNA polymerase

 

iii) DNA pol I lacks 5’→ 3’ exonuclease activity

 

D) T7 RNA polymerase

 

iv) Thermostable DNA polymerase used in PCR

 

E) S1 nuclease

 

v) Lack  5’→ 3’ exonuclease activity

#Part-A Aptitude & General Biotechnology
  1. A-v, B-i, C-ii, D-i, E-iii, 
  2. A-iv, B-iii, C-i, D-ii, E-v, 
  3. A-ii, B-iv, C-v, D-iii, E-i, 
  4. A-iii, B-v, C-iv, D-i, E-ii, 
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TLS Online TPP Program

#Question id: 14333

#Part-B Specialized Branches in Biotechnology

A cylindrical bioreactor of diameter 3 m has four baffles. A Rushton turbine mounted in the reactor has a diameter one third the tank diameter and is operated at a speed of 90 rpm. The density of the fluid is approximately I g cm-3. The reactor is used to culture an anaerobic organism that does not require gas sparging. The broth can be assumed Newtonian. As the cells grow, the viscosity of the broth increases. when the viscosity is 1000 times greater than water, estimate the power required to achieve turbulence.
TLS Online TPP Program

#Question id: 14334

#Part-B Specialized Branches in Biotechnology

Laboratory-scale fermenters are usually mixed using small stirrers with electric motors rated between 100 and 500 W. One such motor is used to drive a 7cm turbine impeller in a small reactor containing fluid with the properties of water. The stirrer speed is 900 rpm. Estimate the power requirements for this process.
TLS Online TPP Program

#Question id: 14336

#Part-B Specialized Branches in Biotechnology

A cylindrical stirred bioreactor of diameter and height 2 m has a Rushton turbine one-third the tank diameter in size. The bioreactor contains Newtonian culture broth with the same density as water and with viscosity 4 cP. The tank is now aerated. In the presence of gas bubbles, the approximate relationship between ungassed power number (NP)0 and gassed power number (NP)g is: (NP)g =0.5 (NP)0. What maximum stirrer speed is now possible in the sparged reactor? Estimate the mixing time.
TLS Online TPP Program

#Question id: 14338

#Part-B Specialized Branches in Biotechnology

Quasi steady state condition is 
TLS Online TPP Program

#Question id: 14340

#Part-B Specialized Branches in Biotechnology

Which bioreactor consists of a multi-layered bag instead of vessel 
TLS Online TPP Program

#Question id: 14369

#Part-B Specialized Branches in Biotechnology

If the value of the determinant

  

then the value of