There are some typical enzyme used in the manipulation of nucleic acid;

ENZYMES	USE IN NUCLEIC ACID MANIPULATION
A) klenow	i) DNA-dependent RNA polymerase
B) T4 DNA polymerase	ii) Single-strand specific nuclease
C) Taq DNA polymerase	iii) DNA pol I lacks 5’→ 3’ exonuclease activity
D) T7 RNA polymerase	iv) Thermostable DNA polymerase used in PCR
E) S1 nuclease	v) Lack  5’→ 3’ exonuclease activity

#Question id: 5248

#Part-A Aptitude & General Biotechnology

Which of the following is a description of generalized transduction?

#Question id: 5249

#Part-A Aptitude & General Biotechnology

What is the best explanation for why Bernard Davis didn't observe generalized transduction in his U tube experiments?

#Question id: 5250

#Part-A Aptitude & General Biotechnology

A researcher would like to map the location of galE and trpA genes in a new species of bacterium that appears to be closely related to E. coli. He decides to use cotransduction, and generatesnappropriate donor and recipient strains. He is disappointed when cotransduction is not seen in his experiement. What is the most reasonable explanation for this situation?

#Question id: 5251

#Part-A Aptitude & General Biotechnology

What do transduction, transformation, and conjugation have in common?

#Question id: 5252

#Part-A Aptitude & General Biotechnology

Which type(s) of genetic transfer lead to incorporation of new DNA into the bacterial chromosome?

I. Conjugation mediated by F factor

II. Hfr mediated conjugation

III. Transduction

IV. Transformation

#Question id: 5253

#Part-A Aptitude & General Biotechnology

In a hypothetical situation, the genes for sex pilus construction and for tetracycline resistance are located together on the same plasmid within a particular bacterium. If this bacterium readily performs conjugation involving a copy of this plasmid, then the resultshould be