Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?

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#Question id: 17083

#Part-B Specialized Branches in Biotechnology

Fusion of two cells produces a single cell with two nuclei one from each of the cells entering fusion; such a cell is called as 

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Which type of pumps inhibits digitoxin in the membranes of heart cells, leading to increased myocardial contraction?

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#Question id: 5632

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In the egg,_______ contains proteolytic enzymes that are released by exocytosis after fertilization and are important for preventing polyspermy.

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#Question id: 1559

#Part-A Aptitude & General Biotechnology

Which of cytokine is responsible for Class Switch IgM into IgE

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#Question id: 422

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The flow of material through a reaction pathway usually depends on ________.