Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

How primer concentration leads to decrease in PCR efficiency?

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#Question id: 18370

#Part-B Specialized Branches in Biotechnology

Corn, wheat, soybean, peas, and beans are few crops which are used as hyperaccumulators as they can 

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#Question id: 14143

#Part-B Specialized Branches in Biotechnology

The higher is the score in the alignment _________

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#Question id: 21735

#Part-B Specialized Branches in Biotechnology

Given the following equation (2A+B  3C). Which of the following correctly displays the rate of the reaction?

I. -Δ[A]/2Δt

II. Δ[B]/Δt

III. Δ[C]/3Δt

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#Question id: 14121

#Part-A Aptitude & General Biotechnology

Which of the following is not the classification of SCOP?

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#Question id: 306

#Part-B Specialized Branches in Biotechnology

The force responsible for dissolution of ionic compound in water is_________