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TLS Online TPP Program

#Question id: 13057

Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
How primer concentration leads to decrease in PCR efficiency?

#Part-A Aptitude & General Biotechnology
  1. Primer concentration decreases with time
  2. Binding specificity of the primer reduces over cycles
  3. Base pairing between the primers reduces their availability
  4. Excess template leads to competition for primer
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TLS Online TPP Program

#Question id: 12820

#Part-A Aptitude & General Biotechnology

The length of a room floor exceeds its breadth by 20 m. The area of the floor remains unaltered when the length is decreased by 10 m but the breadth is increased by 5 m. The area of the floor (in square metres) is :
TLS Online TPP Program

#Question id: 12821

#Part-A Aptitude & General Biotechnology

A street of width 10 metres surrounds from outside a rectangular garden whose measurement is 200 m × 180 m. The area of the path (in square metres) is
TLS Online TPP Program

#Question id: 12822

#Part-A Aptitude & General Biotechnology

In measuring the sides of a rectangle, there is an excess of 5% on one side and 2% deficit on the other. Then the error percent in the area is
TLS Online TPP Program

#Question id: 12823

#Part-A Aptitude & General Biotechnology

A lawn is in the form of a rectangle having its breadth and length in the ratio 3 : 4. The area of the lawn is 1/12 hectare. The breadth of the lawn is
TLS Online TPP Program

#Question id: 12824

#Part-A Aptitude & General Biotechnology

The area of a rectangle is thrice that of a square. The length of the rectangle is 20 cm and the breadth of the rectangle is 3/2 times that of the side of the square. The side of the square (in cm) is
TLS Online TPP Program

#Question id: 12881

#Part-A Aptitude & General Biotechnology

If the height of a cylinder is increased by 15 per cent and the radius of its base is decreased by 10 per cent then by what percent will its curved surface area change?