Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following true about real time PCR?

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#Question id: 3675

#Part-A Aptitude & General Biotechnology

Which describes the role of primase during replication?

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#Question id: 11216

#Part-B Specialized Branches in Biotechnology

When two excitatory postsynaptic potentials (EPSPs) occur at a single synapse so rapidly in succession that the postsynaptic neuron's membrane potential has not returned to the resting potential before the second EPSP arrives, the EPSPs add together producing ________.

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#Question id: 11288

#Part-B Specialized Branches in Biotechnology

Below is a table showing the number of species and actual food web links observed in three different ecosystems. By calculating connectance as a measure of relative complexity of food webs, which of the following ecosystem are more complex? 

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#Question id: 14155

#Part-B Specialized Branches in Biotechnology

Which of the following statement is correct for BLAST?

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#Question id: 19228

#Part-B Specialized Branches in Biotechnology

A physical gene transformation method that can be used only with plant cell protoplasts that can be regenerated into viable plants