Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Efficiency during ligation of blunt ended insert and vector cannot be cannot be enhanced by means of…..

#Question id: 5720

#Part-B Specialized Branches in Biotechnology

Which two of the following are the CORRECT statements ?

P. Nondisjunction in the parental meiosis is not essential to produce diploid organisms

Q. Nondisjunction in the parental meiosis is essential to produce aneuploid organisms

R. Nondisjunction in the parental meiosis is essential to produce hexaploid organism

S. Nondisjunction in the parental meiosis is essential to produce tetraploid organisms

#Question id: 5721

#Part-B Specialized Branches in Biotechnology

There is paracentric inversion heterozygote ABCDEFG/ABFEDCG involved in double recombination during meiosis I within inverted region result in

A. dicentric and an acentric chromosome in meiosis I as the chiasmata get terminated.

B. appearance of deletion in both the cross over product

C. all gamete are viable

D. nonviable gametes from crossover products.

Which of the above statements are correct?

#Question id: 5724

#Part-B Specialized Branches in Biotechnology

Species I is diploid (2n  = 8) with chromosomes

AABBCCDD; related species II is diploid (2n = 8) with chromosomes MMNNOOPP. Individuals with the following sets of chromosomes represent what types of chromosome mutations?

A-   AAABBCCDD        B-   AABBCCDDMNOP

C-   AAABBCCDDD

#Question id: 5724

#Part-A Aptitude & General Biotechnology

Species I is diploid (2n  = 8) with chromosomes

AABBCCDD; related species II is diploid (2n = 8) with chromosomes MMNNOOPP. Individuals with the following sets of chromosomes represent what types of chromosome mutations?

A-   AAABBCCDD        B-   AABBCCDDMNOP

C-   AAABBCCDDD

#Question id: 5730

#Part-B Specialized Branches in Biotechnology

In a family, father is homozygous dominant (AA) for a gene A and his wife is homozygous for its ressive allele (aa) showing albino phenotype. It was surprising that their child showed the albino phenotype. Which of the following phenomenon can explain the phenotype?

#Question id: 5732

#Part-B Specialized Branches in Biotechnology

Consider a plant, such as maize, heterozygous for a paracentric inversion. The inversion is very small and a synapsed inversion loop does not occur in any of the cells. Which of the following statement is correct?