Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Efficiency during ligation of blunt ended insert and vector cannot be cannot be enhanced by means of…..

#Question id: 5693

#Part-B Specialized Branches in Biotechnology

Type of nondisjunction leads to regions of tissue with different chromosome constitutions, a condition known as mosaicism is due to

#Question id: 5694

#Part-A Aptitude & General Biotechnology

In anaphase I, the chromosomes separate in  three different ways , which of the following way for chromosome saparation leads to nonviable gamete formation

#Question id: 5694

#Part-B Specialized Branches in Biotechnology

In anaphase I, the chromosomes separate in  three different ways , which of the following way for chromosome saparation leads to nonviable gamete formation

#Question id: 5695

#Part-A Aptitude & General Biotechnology

Triploids are usually autopolyploids. They arise spontaneously in nature, but they can be constructed by geneticists from the cross of a 4n (tetraploid) and a 2n (diploid). Triploids are characteristically sterile due to

#Question id: 5695

#Part-B Specialized Branches in Biotechnology

Triploids are usually autopolyploids. They arise spontaneously in nature, but they can be constructed by geneticists from the cross of a 4n (tetraploid) and a 2n (diploid). Triploids are characteristically sterile due to

#Question id: 5696

#Part-A Aptitude & General Biotechnology

Abnormality during egg formation as a nondisjuction in anaphase I of single set of chromosome, but spermatogenesis is normal will produced resulting progeny would be