Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

In blue white screening insertional activation of Beta-D-galactosidase will give rise to

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#Question id: 372

#Part-A Aptitude & General Biotechnology

Carbon dioxide in the atmosphere dissolves with the raindrops. The pH of raindrops is ________.

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#Question id: 11880

#Part-A Aptitude & General Biotechnology

The HCF and LCM of two numbers are 21 and 84 respectively. If the ratio of the two numbers is 1 : 4, then the largest of the two numbers is

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#Question id: 773

#Part-A Aptitude & General Biotechnology

Which of the following describes the function of an enzyme known as Dicer?

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#Question id: 14104

#Part-B Specialized Branches in Biotechnology

All the following are nucleotide sequence databases except

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#Question id: 4916

#Part-A Aptitude & General Biotechnology

Kappa particles indicate