Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

In blue white screening insertional activation of Beta-D-galactosidase will give rise to

TLS Online TPP Program

#Question id: 18778

#Part-A Aptitude & General Biotechnology

which of the following technique provides an unusually powerful technology for the quantitative analysis and sorting of cell populations labelled with one or more fluorescent antibodies.

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#Question id: 18779

#Part-A Aptitude & General Biotechnology

Flow-cytometric analysis for the detection and measurement of subpopulations of leukocytes, including those of leukaemia, is usually performed using monoclonal antibodies. Why is this the case?

Choose whether each of the following statements is true 

a) Indirect immunofluorescence is a more sensitive technique than direct immunofluorescence

b) Most antigens induce a polyclonal response. 

c) For precipitation to occur, both antigen and antibody must be multivalent.

d) ELISA tests using chemiluminescence are more sensitive than chromogenic ones and precipitation tests are more sensitive than agglutination tests

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#Question id: 18780

#Part-A Aptitude & General Biotechnology

Which of the following does not participate in the formation of antigen-antibody complexes?

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#Question id: 18781

#Part-A Aptitude & General Biotechnology

In which of the following case a large lattice is formed?

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#Question id: 18782

#Part-A Aptitude & General Biotechnology

The concentration of a small amount antibodies 2 μg antibody/ml can be detected

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#Question id: 18783

#Part-A Aptitude & General Biotechnology

Sensitivity of various immunoassays are given match them correctly

Assay	Sensitivity* (μg antibody/ml)
a) Mancini radial immunodiffusion	i) 2
b) Ouchterlony double immunodiffusion	ii) 20–200
c) Immunoelectrophoresis	iii) 20–200
d) Rocket electrophoresis	iv) 10–50